Our laboratory's focus is on the causes, diagnosis and future treatments of Alzheimer disease. We directly measure the pathophysiology of Alzheimer disease in humans using multiple techniques and perform in vitro cell culture experiments. Our group uses a wide variety of assays and techniques from…
more the most basic applications, such as quantitative measurement labeled peptides to clinical translational studies in diagnostic and therapeutic biomarkers for Alzheimer's disease. Ongoing studies include: 1) In Vivo metabolism of Aβ in Alzheimer's Disease: We have pioneered a technique to measure amyloid-beta metabolism in humans. Ongoing studies in Alzheimer's disease and controls will address the hypothesis that there is a change in amyloid-beta metabolism in people who develop Alzheimer's disease compared to people who do not. 2) Familial Adult Children Study: We are investigating the changes that occur in autosomal dominant Alzheimer disease; including structural changes by MRI, pathological changes by PET-PIB, functional changes by Clinical Dementia Rating and neuropsychometric testing, and pathophysiological changes in CSF biomarkers and CNS protein production and clearance rates. 3) Pharmacodynamic response of proposed disease modifying therapies for Alzheimer disease are tested by measuring the production, clearance and steady-state levels of the targeted proteins, including amyloid-beta. These studies quantitate targeted activity of therapeutics and provide evidence that these compounds are effective in humans. 4) CNS derived proteomics and measurements: We are currently investigating multiple other CNS derived proteins and are developing methods to measure protein metabolism profiles in humans using highly sensitive nano-flow mass spectrometry and in vivo labeling techniques.
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